Orchid seed orchid seeds orchid seed germination asymbiotic orchid seed germination

Orchid seed orchid seeds orchid seed germination asymbiotic orchid seed germination

Hi Nadine!

Nadine Vreeland


The Orchid Seedbank Project
PO Box 7042
Chandler, AZ 85246


Hyponex Fertilizer

Available for the first time in over a decade, Hyponex fertilizer can be used to make orchid media. NOTE: This serves only as the salt base; additional components (agar, sugar, vitamins, etc.) will be required. Recommended for those that make their own media.

US $.90 per 5-gram pouch

US$8 for (10) 5-gram pouches

O156 replate medium

Hot stuff- too hot for most orchids at full strength! Use at 70% strength, or use our OspEx 1.0 (above) for best results. O156 at 100% strength is still good for stanhopeas, gongoras, vandas,  and maybe phals- anything with big, fat roots that can tolerate strong media. US$4 per 1 liter pouch. Contains gelling agent.


Modified P668 mother flask medium (P669)

Our improvement on P668: by adding some B852 banana powder, this formulation prevents the dreaded “brown-out” of protocorms- developing plantlets that turn brown for no good  reason. Contains banana, so it’s probably not good for paph germination, but the concentration is low enough that it won’t faze most “robst” paphs. This is what we use  for germination at the OSP, along with Western W2.5 or W3 + coconut. US$6.50 per 1 liter pouch. Contains gelling agent.


Malto-dextrin (media component)

Used at 5-15 grams per liter, malto-dextrin is a glucose polymer that adds carbohydrates without the osmotic impact of mono- and disaccharides. To be used in addition to other media components  (salts, vitamins, etc.) $2 per 50 gram pouch.

Banana powder (media component)

Used at 5-25 grams per liter, banana powder is just what it sounds like- freeze-dried bananas, ground into a fine powder. Not as useful for germination as it is for replate. May be used at ~5  grams/liter for mother flask (as in our P669, above), but not recommended for germination of paphs. To be used in addition to other media components (salts, vitamins, etc.) $5 per 50 gram  pouch.

OspEx 1.0 replate medium.

Designed and formulated to meet our exacting requirements for maintenance of orchid cultures, OspEx 1.0 is the result of years of research and thousands of flasks. This is the same formulation  we use for 99% of our replate work. Reduced osmotic strength in conjunction with enhanced nutrition for extended durations in culture, this formulation has proven to be reliable across a  broad range of genera. US$4 per 1 liter pouch. Contains gelling agent.

Bulk pack of 10 liters: US$35.

(Note: You will need  a balance that reads to 0.1 g for this product.)

OspEx 2.0 replate medium.

The latest and greatest from our labs. Five years in the making, it’s taken us a while to figure out precisely how to get the stuff made and in the hands of growers. Offering improved performance in a broad variety of genera, version 2.0 now makes up 95% of what we use here in the lab today.

US$4.50 per 1 liter pouch. Contains gelling agent.


P668 mother flask medium.

The original seed germination medium. Effective on a wide array of tropical epiphytic and terrestrial genera, this is the old standby based on Murashige and Skoog salt base with 20 grams of  sugar and all the other goodies (inositol, peptone, B vitamins- the whole kitchen sink is in there). Inexpensive and reliable. US$3.50 per 1 liter pouch. Contains gelling agent.

N1 medium

Originally designed for use with Nepenthes (tropical pitcher plants), it turns out this formulation works quite well with tropical orchids and paphiopedilums in particular. I’ve not had  a chance to experiment around too much with it, so I’m still looking for feedback as to its uses and utility.

Very good with Nepenthes, and quite good with paphs. May also work with strange temperate terrestrials, including some cyps. Note: We’ve been getting very good feedback about N1 for use with paphs.

This is a complete formulation with gelling agent, carbohydrate source, and organic and inorganic nitrogen sources. No activated charcoal. $6 per 1 liter pre-pack.

Iron EDDHA (media component)

EDTA is a good chelant for adding iron to plant tissue culture media, but EDDHA is better- more stable, and less phytotoxic. This product is sold dry powder; dissolve into final volume of 250  mL distilled water, and store in the refrigerator until used. This may be added to supplement the iron in prepared media (1-5 mL per liter of finished medium), or added as the entire iron  source (10 mL per liter). $5 per packet.